Is this considered retaliation and can I report it/be covered by the EEOC?

Is this considered retaliation. I applied for an interoffice transfer to a different position. I am currently in a position for a public agency and applied for a less stressful position within the company. I was willing to take the pay cut as it is a step down. The transfer was disapproved but ever since my submission my boss constantly rides me, has made false negative entries into my personnel file (which I did not know of until I viewed my personnel file today), canceled previously scheduled training, given me extremely low review marks and denied my annual raise. Please note that this is a new supervisor who just took over 2 weeks prior to my requesting the transfer and had nothing to do with her. Prior to my New supervisor my old Supervisor always praised my work and I never received any negative reviews. The CEO even gave me a "Good Job" on a couple of presentations I had to lead. One was for a local Congressman and another was for High Ranking Military Officials. My coworkers have noticed what is going on and have told me to "Watch out" because my supervisor is up to something. My workplace feels toxic and I feel like i have to walk around on egg shells always looking over my shoulder. Even as I write this I do not know if I will have a job at the end of the week and it has been like this ever since i applied for the other position, 3 months now. Please advise. I'm slowly losing my mind.

Update: I went to work this morning and was informed that my employment has been terminated. Please advise as what my options are at this point.